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. 2019 Jan 25;10:448. doi: 10.1038/s41467-018-08271-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Batf3-dependent CD103+ dendritic cells are required for CD8+ T cells priming. a Brain-infiltrating leukocytes (BIL) and tumor-draining lymph nodes (TDLNs) of Ctrl or FGL2KO tumor-bearing wild-type (WT) mice and FGL2KO tumor-bearing Batf3^−/− mice were analyzed for CD103⁺/CD8a⁺ dendritic cell (DCs) populations on day 7 post tumor implantation. Data are presented as the mean ± S.D. and were analyzed by one-way ANOVA (n = 5–7 per group). b FGL2KO tumor progression in CD11c-DTR mice treated with diphtheria toxin (DT) or PBS. FGL2KO tumor cells were implanted subcutaneously into CD11c-DTR mice on day 1 after injection of DT or PBS. Data are presented as the mean ± S.D. (n = 8 per group). c Survival analysis of Batf3^−/− mice implanted with GL261-FGL2KO tumor cells (n = 7 per group). d FACS assay of CD69 on naive OT-I CD8⁺ T cells co-cultured with DCs isolated from tumors at a ratio of 1:2. Data are presented as the mean ± S.D. (n = 3 mice per group) and were analyzed by t-test. e Abundance and proliferation of CFSE-labeled OT-I cells in brains and TDLNs of GL261-Ctrl-OVA or GL261-FGL2KO-OVA tumor-bearing mice. CFSE-labeled OT-I cells were adoptively transferred to tumor-bearing CD45.1 mice on day 2 after tumor implantation. Brains and TDLNs were collected 5 days later for analyzing the presence and proliferation of the CD45.2⁺CD8⁺ T cell population. Data are presented as the mean ± S.D. (n = 5-6 per group) and were analyzed by t-test. f Representative cytometric analysis of CD8⁺ T cell priming (CD8⁺T-bet⁺Eomes⁻) in BIL and TDLNs at 7 days after tumor implantation. Data are presented as the mean ± S.D. (n = 5 per group) and were analyzed by t-test. g Survival analysis of WT mice implanted with GL261-FGL2KO tumor cells treated with FTY720. Mice were treated with 1 mg/kg FTY720 1 h before tumor-cell implantation and were maintained with drinking water containing 2 μg/mL FTY720 for the duration of the experiment (n = 6 per group). All data are representative of at least two independent experiments. The survival curves were analyzed by Kaplan–Meier analysis and the log-rank test was used to compare overall survival between groups. Significant results were presented as *P < 0.05, **P < 0.01, ***P < 0.001