Fig. 6. KDM4B regulates the expression of integrin αV.

a Reduced expression of integrin αV encoded by ITGAV in KDM4B-knockdown cells. qRT-PCR analysis was performed to evaluate the expression of ITGAV in pLKO, sh4B#1, and sh4B#2 cells, respectively. Cells were either not infected (−Hp) or infected with strain 26695 (+ Hp) at an moi of 50 for 3 h. b, c Silencing of integrin αV and β1 reduces H. pylori-stimulated IL-8 production. AGS cells were transfected with control (si-scr), integrin αV (si-ITGAV), or β1 (si-ITGB1) siRNA and cultured for 24 h, followed by non-infection (−Hp) or infection with H. pylori (+ Hp) at an moi of 50 for 3 h. Total RNAs were prepared and subjected to qRT-PCR for ITGAV and ITGB1 expression (b). IL-8 released into the culture supernatant was measured by ELISA (c). d–f KDM4B is recruited at the ITGAV locus. Chromatin was prepared from pLKO, sh4B#1, or sh4B#2 cells with anti-KDM4B (d), c-Jun (e), or H3K9me3 (f), followed by quantitative PCR analysis. Fold enrichments normalized to IgG are presented. The values are means and SDs of three independent experiments. Statistical significance was evaluated using the Student’s t-test. *p < 0.05; **p < 0.01