Fig. 2.

Posterior, hh-gal4-induced overexpression of Dlish causes Dachs-independent overgrowth. (A–C) Adult wings and ratios of posterior to anterior area (p/a) ±SD. (A) Wild type, with line showing subdivision into anterior and posterior (hh-gal4-expressing) regions. (B) hh-gal4 UAS-dlish-FLAG. (C) hh-gal4 > dlish-FLAG in hypomorphic d¹/d^GC13 background. The p/a ratio is significantly higher in both compared with wild type using a two-tailed Mann–Whitney U test. (Magnification: 50×.) (D–F) Example late third instar imaginal wing discs and average ratios of p/a area ±SD in wing pouch and hinge regions from N discs. (D) hh-gal4 UAS-GFP; posterior marked with GFP (green) and anterior with anti-Ci (magenta); lines show regions measured. (E and F) hh-gal4 UAS-dlish-FLAG (posterior marked with anti-FLAG) in wild type (E) and d^GC13/d^GC13 (F) wing discs. The p/a ratio is significantly higher in both compared with hh-gal4 UAS-GFP using either a two-tailed Mann–Whitney U test or two-tailed t test. (Scale bar [D–F]: 100 μm.)