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. 2019 Jan 3;116(4):1319–1324. doi: 10.1073/pnas.1811891116

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Copyright © 2019 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 3. — Characterization of the binding between Dlish and Ex. (A) Reciprocal co-IP between V5-tagged Dlish and FLAG-tagged Ex made in S2R+ cells. (B) Domain organization of Ex. (C) HA-tagged Dlish co-IPs with full-length Ex-FLAG and ExC-FLAG (aa 654–1427) but not ExN-FLAG (aa 1–653) in S2R+ cells. (D) HA-tagged Dlish co-IPs with ExC2-FLAG (aa 912–1164) and ExC3-FLAG (aa 1168–1427) but not ExC1-FLAG. (aa 654–911). (E) GST-purified Dlish co-IPs with in vitro translated (IVT) FLAG-ExC3 but not FLAG-ExC1 or FLAG-ExC2. (F) Co-IP of HA-tagged Dlish is lost when candidate SH3-binding domains (SH3bd) 1 and 3 are both removed from ExC3-FLAG. Matches of the candidate domains to class I and II SH3-binding domains are shown below; + = basic, X = non-G hydrophobic, x = any, blue = mismatch. (G) Co-IP of HA-tagged Ex is lost when SH3 domains 2 and 3 are both removed from Dlish-FLAG.