Fig. 4.

Loss of Dlish increases Ex in wing imaginal discs. (A) Increased subapical Ex (magenta, grey in A′) in dlish⁴⁵⁰⁶ homozygous clones (loss of green). (B–D) Increased subapical Ex (magenta, grey in B′–D′) after driving UAS-dlish-RNAi and UAS-GFP dorsally with ap-gal4 (B), or posteriorly with hh-gal4 in wild type (C) or ft^fd homozygotes (D), marked by loss of Dlish (green). Cross-sections show subapical regions. (E) Example Western IB (E) and quantification from multiple IBs (E′) of Ex levels in extracts from w¹¹¹⁸ control, phenotypically wild-type control 2 (Bloomington 51324) and dlish mutant discs. Quantifications were normalized to w¹¹¹⁸ lanes from the same IB, and average changes (three extracts for each) compared using paired two-tailed t tests; NS, not significant; error bars = ±SD. (F and G) ex-lacZ expression (anti-βgal, magenta, gray in F′ and G′) is not altered by posterior, hh-gal4-driven UAS-dlish-RNAi (F, posterior marked by absence of green Ci; detail shows cross-section through disc) or by dlish⁰⁴ mutant clones (G, marked by loss of green). (H) Expression of the Yki target Diap1 (magenta, grey in H′) is decreased by posterior, hh-gal4-driven UAS-dlish-RNAi in ft^fd homozygote. (Scale bars: 50 μm.)