Fig. 5.

RNA degradosomes mediate tRNA decay in vitro. (A) Coomasie-stained gel image of Flag-RNase E immunoprecipitated fraction. Indicated bands were identified through peptide mass spectrometry. (B) In vitro tRNA-Tyr decay assay. Time course of tRNA-Tyr resolved in 10% TBE-UREA gel stained with SYBR Gold at the indicated time. Upper and Lower show reactions with degradosomes from the WT and from the PNPase catalytic mutant, respectively. (C) Decay curves of tRNA-Tyr from WT and ΔthiI mutant strains based on data shown in B.