Fig. 1.

Pluripotent cells lack the IFN-I response observed in differentiated cells. (A) Analysis of the transcriptional variation between FFs, iPSCs, and rederived foreskin fibroblasts (reFFs) by MDS. (B) Western blot of cells described in A either mock treated or transfected with viral RNA (pppRNA) or treated with IFN-beta (IFNB). Blots depict levels of RIG-I, IFIT1, and ACTIN. (C) Same conditions as B in which RNA was analyzed by quantitative real-time PCR for IFNB mRNA expression, represented as fold change and normalized to mock-treated samples.