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. 2019 Jan 26;26:13. doi: 10.1186/s12929-019-0506-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — In vitro function of AMC-HN-8 and TU177 cells with overexpression of ZNF667-AS1. a. MTS assays of cells transfected with pcDNA3.1 empty vector or pcDNA3.1-ZNF667-AS1, showing that cell transfected with pcDNA3.1-ZNF667-AS1 proliferate slower than that transfected with empty vector at the indicated time points. b. Clone formation assays of cells with or without overexpression of ZNF667-AS1. Cells overexpressing ZNF667-AS1 formed less clones than cells transfected with pcDNA3.1 empty vector. c. Transwell migration assays of AMC-HN-8 and TU177 cells were performed with or without ZNF667-AS1 overexpression. The migration ability of cells overexpressing ZNF667-AS1 decreased than cells transfected with pcDNA3.1 empty vector. d. Transwell invasion assays of AMC-HN-8 and TU177 cells were performed with or without ZNF667-AS1 overexpression. The invasion ability of cells overexpressing ZNF667-AS1 decreased than cells transfected with pcDNA3.1 empty vector. pcDNA3.1: the vector control group; ZNF667-AS1: ZNF667-AS1 over-expression group. The difference was statistically significant, **P < 0.01