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. 2019 Jan 26;26:13. doi: 10.1186/s12929-019-0506-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — The regulation mechanism of ZNF667-AS1 and ZNF667. a. The RNA of ZNF667-AS1 was mainly located on the nucleus. b. The mRNA of ZNF667 was mainly located on the nucleus. c. Overexpression of ZNF667-AS1 increased the expression of ZNF667 in mRNA level, detected by qRT-PCR. d. Overexpression of ZNF667 didn’t increase the expression of ZNF667-AS1 in mRNA level, detected by qRT-PCR. e. Overexpression of ZNF667-AS1 increased the expression of ZNF667 in protein level, detected by western blot. f and g. Overexpression of ZNF667-AS1 increased the expression of EMT associated markers. h and i. Overexpression of ZNF667 increased the expression of EMT associated markers partially. In Fig. 7c-i, pcDNA3.1: the vector control group; ZNF667-AS1: ZNF667-AS1 over-expression group; ZNF667: ZNF667 over-expression group. The difference was statistically significant, **P < 0.01