Extended data Table 1. Screened antibodies by CyTOF for binding of B. schlosseri cells.
A screen of 49 antibodies that would cross-react with B. schlosseri cells and could potentially differentiate cellular populations. Symbol: represents the element, mass: is the elemental isotope mass, antigen: is the human antigen against which the antibody was produced, antibody clone, and antibody supplier. CyTOF column represents whether the B. schlosseri cell population was positive or not, low means less than 1% of cells were positive. FACS column represents whether there was binding by the same antibody clone by flow cytometry.
| Symbol | Mass | Panel1 antigen |
Clone | Supplier | CyTOF | FACS | Panel2 antigen |
Clone | Supplier | CyTOF | FACS |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Pd/Cd | 110–114 | CD3 | S4.1 | Invitrogen | Yes | No | |||||
| In | 113 | CD7 | M-T701 | BD | Yes | No | |||||
| In | 115 | CD45 | HI30 | Biolegend | Yes | No | |||||
| La | 139 | CD57 | HCD57 | Biolegend | Yes (low) | Yes (~14%) | CD2 | RPA-2.10 | Biolegend | Yes (low) | No |
| Pr | 141 | NKp46 | 195314 | R&D system | No | CD61 | VI-PL2 | Biolegend | No | ||
| Nd | 142 | CD48 | TU145 | BD | No | ||||||
| Nd | 144 | CCR5 | HEK/l/85a | Biolegend | Yes | N/A | CD94 | DX22 | Biolegend | Yes (low) | N/A |
| Nd | 145 | LILRB1 | 292319 | R&D | No | ||||||
| Nd | 146 | CD309 | 89106 | BD | No | ||||||
| Sm | 147 | CD8 | RPA-T8 | Biolegend | No | ||||||
| Nd | 148 | KIR3DL1 | DX9 | BD | No | CRTAM | Cr24.1 | Biolegend | No | ||
| Sm | 149 | DNAM1 | DX11 | BD | No | ||||||
| Eu | 151 | NKG2D | 1D11 | Biolegend | No | CD84 | CD84.1.21 | Biolegend | No | ||
| Sm | 152 | TNFR2 | 22235 | R&D | No | ||||||
| Eu | 153 | NKG2C | 134522 | R&D | Yes (low) | N/A | |||||
| Sm | 154 | NKp44 | P44–8 | Biolegend | No | Notchl | MHN1–519 | Biolegend | No | ||
| Gd | 155 | CRACC | 162.1 | Biolegend | No | ||||||
| Gd | 156 | KIR2DL3 | 180701 | R&D | No | ||||||
| Gd | 158 | CD161 | HP-3G10 | Biolegend | No | clecl2A | 50C1 | Biolegend | No | ||
| Tb | 159 | CDllc | Bul5 | Biolegend | No | ||||||
| Gd | 160 | NKp30 | 210845 | R&D | No | 2B4 | Cl.7 | Beckman | No | ||
| Dy | 161 | CD15 | W6D3 | Biolegend | No | CD4 | RPA-T4 | Biolegend | Yes | No | |
| Dy | 162 | CD49d | 9F10 | Biolegend | Yes | Yes (~28%) | |||||
| Dy | 163 | CD16 | 3G8 | Biolegend | No | KIR2DLS1 | EB6.B | Beckman | No | ||
| Er | 166 | TIGIT | M BSA43 | eBioscience | No | KIR3DL2 | DX31 | Gift from Dr. Lanier | No | ||
| Er | 167 | CD27 | 0323 | Biolegend | No | CCR7 | G043H7 | Biolegend | No | ||
| Er | 168 | KIR2DL1 | 143211 | R&D | No | CCR2 | 48607 | BD | Yes | No | |
| Er | 170 | CDlla | Hllll | Biolegend | No | CDllbact | CBRM1/5 | Biolegend | No | ||
| Yb | 172 | KIR2DL4 | 181703 | R&D | No | CD34 | 8G12 | BD | Yes (low) | N/A | |
| Yb | 173 | CD33 | WM53 | Biolegend | No | ||||||
| Yb | 174 | CD 144 | TEA1/31 | Beckman | No | NKG2A | Z199 | Beckman | No | ||
| Lu | 175 | KLRG1 | 13F12F2 | ThermoFisher | No | ||||||
| Yb | 176 | CD56 | NCAM16.2 | BD | Yes (low) | N/A | |||||
| Ir | 191/193 | DNA | Sigma | All cells | DNA | Sigma | All cells | ||||
| Pt | 195 | cisplatin | Sigma | No | Sigma |