Fig. 3.

a Schematic representation depicting the use of clickable ABPPs. b Chemical structures of RFA and RCA. c Schematic overview of the fluorescence polarization assay using RFA as an ABPP for high-throughput inhibitor discovery. d Structures of first-generation ‘clickable’ PAD-targeted ABPPs. Labeled probes are subjected to click-chemistry using fluorescein reporters through a post-inactivation strategy. e Structures of second-generation ‘clickable’ PAD-targeted ABPPs. Probe-labeled proteins are subjected to click-chemistry using TAMRA azide reporter through a post-inactivation strategy