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. Author manuscript; available in PMC: 2019 Apr 24.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2018 Jul 26;102(5):1569–1576. doi: 10.1016/j.ijrobp.2018.07.192

Figure 3. Treatment with palbociclib before a single dose of sub-total-body irradiation increases the number of surviving crypts in the small intestine.

Figure 3.

Mice were treated with palbociclib or vehicle 28 and 4 hours before 0, 12, 13, 14 and 15 Gy SBI. Proximal small intestines were harvested for histology 96 hours after irradiation. (A) Representative tissue sections of the small intestine 96 hours after 13 Gy SBI stained with an anti-BrdU antibody. Scale bars, 100 μm. (B) Quantification of surviving crypts 96 hours after various doses of SBI. To quantify the number of surviving crypts, tissue sections were stained for BrdU, which labels proliferating crypt cells. Data are presented as means ± SEM (n =3 mice group). *P < 0.05 by two-way ANOVA with Bonferroni’s post hoc test.