Table 2. Fluorescence Properties of H-Telo DNA ONs 3–5 and Respective Duplexes in Aqueous Buffer.
ON sample |
λem (nm) |
Φa | Φrb |
τavc (ns) |
ON sample |
λem (nm) |
Φa | Φrb |
τavc (ns) |
ON sample |
λem (nm) |
Φa | Φrb |
τav c (ns) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
3 in NaCl | 424 | 0.053 | 27 | 1.09 | 4 in NaCl | 428 | 0.093 | 93 | 1.40 | 5 in NaCl | 428 | 0.062 | 31 | 0.89 |
3 in KCl | 429 | 0.030 | 15 | 0.60 | 4 in KCl | 428 | 0.022 | 11 | 0.79 | 5 in KCl | 423 | 0.043 | 22 | 0.73 |
3·7 in NaCl | 426 | 0.002 | 1 | n.d. | 4·7 in NaCl | 426 | 0.001 | 1 | n.d. | 5·7 in NaCl | 428 | 0.002 | 1 | n.d. |
3·7 in KCl | 432 | 0.002 | 1 | n.d. | 4·7 in KCl | 428 | 0.002 | 1 | n.d. | 5·7 in KCl | 426 | 0.002 | 1 | n.d. |
Standard deviation for quantum yield (Φ) of GQs is ≤0.006, and that of duplexes is ≤0.0002.
Φr = relative quantum yield with respect to the quantum yield of the duplex in the respective ionic conditions.
Standard deviation for lifetime (τav) is ≤0.06 ns. n.d. = not determined. Reliable lifetime values could not be obtained for duplexes as they were very weakly fluorescent.