Figure 5. LPA induced oligodendrocyte cell death in vitro through LPA₂ signaling on microglial cells.

(A) Quantification of MBP⁺ cells on primary cultured WT and LPA₂ null oligodendrocytes exposed to 1 μM LPA for 24h. (*p<0.05; n=8 biological replicates for vehicle and n=7 biological replicates for LPA in both groups). (B) Quantification of MBP⁺ cells on primary cultured WT oligodendrocytes exposed for 24h to LPA-stimulated microglial-conditioned medium (MCM) from WT or LPA₂ deficient mice. (*p<0.05, #p< 0.01 vs. control; n=8 biological replicates per group). (C-F) Representative micrographs showing MBP⁺ cells exposed to WT (C, E) or LPA₂ null (D, F) MCM in control (C, D) or LPA (E, F) conditions. Error bars indicate SEM. Scale bar=100 μm