FIGURE 1.

Depletion of fat mass and obesity-associated protein (FTO) in spermatogonia via CRISPR/Cas9 system. (A) Schematic diagram of sgRNAs designed for FTO knockout. Sequences of modified Fto loci in FTO-KO cells. (B) Western blot analysis of FTO expression in wild-type cells and FTO-KO cells. β-actin was used as the loading control. (C) m⁶A dot blot analysis of wild-type cells and FTO-KO cells. Methyl blue stain was used to eliminate the difference in RNA amount.