FIGURE 4.
Effect of FTO depletion on cell cycle. (A) Cell cycle of FTO-KO and wild-type cells were analyzed by flow cytometry. (B) Percentage of G2 stage cells were shown in column diagram. Data were represented by the mean ± SEM, n = 3, ∗∗p < 0.01. (C) Relative mRNA level of genes involved in G2/M transition. Data were represented by the mean ± SEM, n = 3, ∗∗p < 0.01. (D) Western blot analysis of proteins involved in G2/M transition. β-actin was used as the loading control.