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. 2019 Jan 21;9:3095. doi: 10.3389/fimmu.2018.03095

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Copyright © 2019 Du and Wei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ways to expand NK cells. Expanded NK cells can be generated from PMBC or CB: (1) culture with cytokines and anti-CD3 without feeder cells, (2) culture with cytokines and irradiated EBV-LCL, and (3) GMP-compliant countercurrent elutriation, and (4) using an APC K562-based system.