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. 2019 Jan 21;12:509. doi: 10.3389/fncel.2018.00509

Figure 1.

Figure 1

Brain derived neurotrophic factor (BDNF) expression in transgenic spinocerebellar ataxia type 1 (SCA1) mice. (A–B) Relative Ataxin-1 (ATXN1) (A) and BDNF (B) expression in the human cerebellar cortex [polyglutamine (polyQ) sequence/SCA1 status unknown] obtained from the transcriptome data at Allen Brain Institute. Raw reads per kilobase million (RPKM) values were normalized to the highest and lowest values and data was averaged when multiple patient samples fell within the same postnatal year or same post conception week. Open circles represent single patient samples and closed circles represent average expression (used when possible). Shaded areas mark pre-natal period. (C–E) Reverse transcription and quantitative polymerase chain reaction (RT-qPCR) using cerebellar extracts from (C) early stage (5–7 weeks) and (D) late stage (18–24 weeks old) ATXN1[82Q] mice. Results are normalized using 18S RNA and age-matched wild-type (WT) littermates. (E) RT-qPCR using cerebellar extracts from ATXN1[82Q] mice and mice in which astroglialnuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) is inhibited early indisease [ATXN1[82Q]; aIKKβ KO]. Results are normalized using 18S RNA and age-matched WT littermates. For (C–E) error bars = SEM. Student’s t-test P values. Each dot represents a biological sample.