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. 2019 Jan 21;9:733. doi: 10.3389/fgene.2018.00733

FIGURE 4.

FIGURE 4

Zebrafish maf coincides with SUA2 enhancer activity. (A–C) Whole mount in situ hybridization with a riboprobe detecting zebrafish maf was performed on wild type embryos at 12, 24, and 48 hpf. maf expression is present in the pronephros at 12 hpf and the proximal tubules at 24 and 48 hpf. (D) The SUA2 SNP region drives expression of GFP in the proximal tubules at 48 hpf. (E) ChIP analysis of the HNF4α binding site at the SUA2 SNP region, a positive HNF4α site at ABCC6 and a negative HNF4α site at B-globin. Binding is shown relative to input chromatin. Bar graphs represent mean from three independent experiments. A one-way ANOVA test indicates a significant difference between the means of the SUA2 SNP region and the negative binding site in B-globin. There is no difference between the positive ABCC6 site and the SUA2 SNP region. Error bars denote standard error of the mean and asterisks indicate significance: ∗p < 0.005; ∗∗p < 0.005, ∗∗∗p < 0.0005.