Fig. 2.

MSKE arrested G0/G1 phase transition in PC-3 cells. (A) Describes the relative percentage of cells in the G0/G1 phase of cell cycle after 12 hours of MSKE treatment, (B) after 24 hours of MSKE treatment; Resveratrol treatment (25 μM) used as a control for G0/G1 arrest. The cells were fixed by adding 400 μl of ethanol and incubated on ice for 15 minutes. The cells were then centrifuged at 1500 rpm for 5 minutes and the pellet was re-suspended in 200 μl propidium iodide (PI) solution containing 50 μg/ml PI (Biotium), 0.1 mg/ml RNase A (Sigma-Aldrich), and 0.05% Triton X-100 (Sigma-Aldrich). The cellometer allows simultaneous acquisition of bright-field and fluorescence images for concentration, size, and viability measurement to measure cell cycle transition. The fluorescence intensity and size measurement were recorded for each cell contained within a data set, which was exported from the software into an Excel file, and imported into FlowJo (Tree Star, Oregon) for cytometric data analysis. Columns mean percentage of populated cells in G0/G1 phase of the cell cycle; bars, ±SE. * for three independent experiments, is the significant difference between treated and control.