Fig. 7.

(A) MSKE inhibits cell growth in E006AA prostate cancer cells. Cell cultures were treated with various concentrations of MSKE (2.5, 5, 10, 20, 40 μg/ml) during different time intervals (24, 48, and 72 hours). At the end of each experiment MTT assays were performed as described in the methods section to estimate cell numbers. (B) MSKE treatment decreases protein expression of Hsp27, Hsp40, Hsp60, hsp70, hsp90, NF-κB, and cyclin D1 in E006AA after 24 hours. (C) Protein array used to examine the effect of MSKE (10, 20 μg/ml) on proteins involved in cell cycle and apoptosis compared to an ethanol control after 24 hours. Results presented as columns represent mean fold-change over ethanol, bars ± SE, *. (D) IHC analysis of Hsp40 was performed in paraffin-embedded tumor samples from control and MSKE-treated mice. Representation of Hsp40 from three IHC samples from tumor specimen. For statistical analyses, an average of three fields of view per sample was used for counting and the average percent positive cells were counted and graphed. Statistical analyses were performed using the Students' t-test with p ≤ 0.05 establishing significance. Values are means ± SE. *, is the significant difference between MSKE treatment and control.