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Thermodynamic stability of the unmodified and cmo5U‐modified RNAs. a) Secondary structures of the four RNAs under investigation. The inset shows the structure of the cmo5U modification. The nitrogen atoms highlighted in blue are 15N labeled. b) Bar graph of the melting temperatures of the hairpin RNAs. c) Bar graph of the free energies at 298 K of the hairpin RNAs.
