Fig. 5.

GC-MS total ion chromatograms of tumor metabolites in colonosphere-xenografted mice with or without fucoxanthin (Fx) treatment. BALB/c-nu/nu mice inoculated with Csps suspension were treated with or without Fx for 3 weeks. The tumor at the end of the experiment was collected, the metabolites were extracted and then analyzed by GC-MS. Peak numbers are indicated as follows: 1, butyric acid; 2, isoleucine; 3, proline; 4, glycine (arrow); 5, succinic acid; 6, fumaric acid; 7, pelargonic acid; 8, alanine; 9, serine; 10, threonine; 11, malic acid; 12, aspartic acid; 13, γ-aminobutyric acid; 14, glutamic acid. The information of tumor metabolites corresponding to each peak number is shown in Table 2. GC-MS conditions are given in Materials and Methods section.