Figure 3.

HoxB8 neutrophils generate ROS upon integrin/FcγR stimulation. (A) HoxB8 neutrophils were or were not subjected to purification by percoll gradient before being stimulated with 100 nM PMA or its vehicle for ROS assays. A representative example is shown. Red symbols, no percoll gradient; blue symbols, with percoll gradient (B–G). ROS generation of percoll‐enriched HoxB8 cells that had or had not been pre‐incubated with DPI prior to stimulation with PMA (B, E), being plated onto pRGD (C, F), or immobilized ICs (D, G). Representative examples (mean ± range) from a minimum of 4 separate experiments are plotted (B–D). Integrated results are plotted normalized to the activated condition (E–G). *P < 0.05; **P < 0.01; statistical analysis was by t test. Results shown in this figure were obtained with HoxB8 neutrophils from a single donor, and reflect those obtained with cells obtained from two other donors on a different plate reader