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. 2018 Nov 21;57(51):16688–16692. doi: 10.1002/anie.201809149

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© 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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a) Densitometry quantification of the appearance of OmpA(W7C)[MTS‐diazirine] crosslinked to Skp vs. irradiation time. Inset: expanded time axis to show full time course using the Hg‐Xe lamp. Lines are exponential fits to the data. Relative intensity of crosslinked product [%] was calculated as the intensity of the crosslinked product band on an SDS‐PAGE gel at time t divided by the intensity at t _final, assuming the maximal yield is achieved when the graph plateaus (see example SDS‐PAGE of the XL reaction (bottom) and Figure S4c). b) Sample heating at irradiation times required to reach 15, 55 and 100 % maximal crosslinked product for the Hg‐Xe and UV LED lamps. n.d.=not determined.