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Enrichment and analysis strategies for MTS‐diazirine and MTS‐TFMD. Irradiation at 365 nm results in crosslinking of bait and target proteins (n.b. low reaction efficiencies mean that uncrosslinked material will remain). Enrichment can be performed using one of three methods (see the text). MS analysis of the peptides is then performed, and the data searched to identify the peptides/residues modified with the crosslinking reagent (with the free thiol capped by reaction with IAA).
