Table 3.
Validation of Differentially Methylated Regions Identified by RRBS With 450K Methylation Array Data.8,a
| Comparison DMR (RRBS) | Δ CpG methylation | Probe_ID | Distance to DMR | t-Test Score | t-Test: P Value | Hyper/Hypostatus | Mann-Whitney: P Value | Kolmogorov-Smirnov Test: P Value |
|---|---|---|---|---|---|---|---|---|
| IUGR-AGA | ||||||||
| chr5:175969381-175969447 | −0.207 | cg22733478 | 0 | −2.080 | 0.040b | Consistent | .014b | .066 |
| chr3:185912389-185912478 | −0.255 | cg24490859 | 0 | −2.623 | 0.010b | Consistent | .005b | .011b |
| LGA-IUGR | ||||||||
| chr7:989199-989229 | 0.248 | cg15914863 | 0 | 1.381 | 0.198 | Consistent | .071 | .058 |
Abbreviations: AGA, appropriate for gestational age; DMR, differentially methylated region; IUGR, intrauterine growth restriction; LGA, large for gestational age; RRBS, reduced representation bisulfite sequencing.
aTable including 3 loci where methylation levels were measured by both methods, the difference in methylation levels between groups, t-test score, and p values.
bSignificance with p-value < 0.05 by t-test.