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B16-F10 cells were incubated for indicated time points with 51.2 μM fluorinated sialic acid analogues or DMSO control. After sample preparation, the CMP–SiaFR (a) and CMP–Sia (b) levels were analyzed using reverse-phase ion pairing chromatography coupled to a triple quadrupole mass spectrometer operating in negative ion mode and presented as their abundance in the nucleotide sugar pool ±SEM (n = 2).
