Figure 14.
(A) Electrochemical detection assay for HIV biomarker IFN-γ that utilized an Ir(III) redox probe. A gold working electrode was functionalized with a molecular beacon containing an IFN-γ-specific aptamer and an oligonucleotide sequence that was complementary to H1. Hybridization of H1 and H2 formed a double helix to which the Ir(III) probe could bind via the major and minor grooves. (B) The redox reaction of [Ir(ppy)2(phen-dione)]+ was achieved by differential pulse voltammetry, which produced a current that was correlated to the presence of IFN-γ. Adapted with permission from ref (145). Copyright 2017 Nature Publishing Group (https://creativecommons.org/licenses/by/4.0/legalcode).