Figure 16.
Membrane strip test that utilizes ECL probe-loaded liposomes for detection of Legionella antigen. Immunoliposomes functionalized with anti-Legionella antibodies were loaded with the ECL probe [Ru(bpy)3]2+ and incubated with a sample. The sample was then allowed to wick up a nitrocellulose strip that had already been impregnated with the Legionella antigen. In samples containing Legionella antigen, the immunoliposome migrated past the nitrocellulose to a glass fiber pad presoaked with SDS, which lysed the liposome and released [Ru(bpy)3]2+. [Ru(bpy)3]2+ was then detected by coreactant ECL. Adapted with permission from ref (150). Copyright 2003 Elsevier.