Figure 8.

VZ185 induces strong and rapid degradation in a time- and dose-dependent manner. (A) Western blot analysis of BRD9, BRD7, and β-actin after treatment of RI-1 cells with six different concentrations of compounds 46 and 51 for 2 and 8 h before harvesting. (B) Chemical structures of 51 (VZ185) and 46. (C) Quantification of protein levels relative to DMSO control after treatment with different concentration of VZ185 and DC₅₀ values. (D) Time-dependent experiment in RI-1 cells after treatment with 10 nM and 100 nM 51 at the desired time points. Intensity values and DC₅₀ were quantified as described in the Experimental Section.