Figure 6. Synthesis of Pdr1 increases in the absence of Bre5.

A. The indicated strains were grown to early log phase and then labeled with the addition of ³⁵S-methionine for 12 minutes. After this labeling period, a large excess of unlabeled methionine was added and time points removed immediately (0 time) or at 35 and 70 minutes. All time points were processed simultaneously to prepared a cell-free protein extract. Anti-Pdr1 immunoprecipitations were then carried out on each time point.