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. 2019 Jan 24;15:1744806918825044. doi: 10.1177/1744806918825044

Figure 5.

Figure 5.

The effect of GLT1 overexpression on CFA-induced hyperalgesia. (A) GLT1-GFP immunostaining in the RVM. (a) Low power image of GLT1-GFP immunostaining. The area in the rectangle is enlarged in (b) to (d). (b) GLT1-GFP staining in high power. (c) GFAP immunostaining. (d) Double immunofluorescence labeling of GLT1-GFP and GFAP illustrating astroglial expression of transfected GLT1-GFP. (B) GLT1-GFP plasmid was transfected into the RVM at four weeks after CFA and its effect on behavioral hyperalgesia was assessed. GLT1 overexpression led to an increase in PWLs (Left) and PW threshold (Right) in both hind paws at five and seven days after gene transfer (Left). Note reversal of hyperalgesia on the inflamed paw. ***p < 0.001 vs. ctrl; +p < 0.05; +++p < 0.001 vs. four-week post-CFA (Day 0). GLT1: glutamate type 1 transporter; GFAP: glial fibrillary acidic protein; GFP: green fluorescent protein.