Fig. 4.

Effect of apoE3 on the kinetics of early phase aggregation of Aβ(1–40). The reaction mixture containing 5 μM Aβ(1–40), 10 μl suspensions of Matrigel-coated beads, 0.3 mg/ml HSA, PBS-NaN₃, 5 μM ThT, and 0 to 0.5 μM apoE3 was incubated at 37 °C with rotation at 1 rpm in the absence of an air-water interface (30 replicate wells in each case). As a positive control, the reaction mixture containing 5 μM Aβ(1–40), 0.3 mg/ml HSA, PBS-NaN₃, and 5 μM ThT was also incubated at 37 °C with rotation at 1 rpm in the presence of an air-water interface (12 replicate wells) (Air). The kinetics of early phase aggregation was analysed by the Kaplan-Meier survival method using the initiation time of fibril growth kinetics as the event of interest (see Fig. 2). The P value was less than 0.05 between all combinations. This data is representative of three independent experiments