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. 2019 Jan 28;18:17. doi: 10.1186/s12934-019-1066-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — SDS-PAGE analysis of rhFGF21 expression following ultrasonication. Total cell protein (TCP), supernatant, and pellet fractions from B. subtilis strains that overexpressed different chaperones at incubation of 24 h were run. Strains B. subtilis CHAP1, CHAP2, CHAP3, CHAP4, CHAP5, CHAP6, CHAP7, CHAP8, CHAP9, CHAP10, and CHAP11 overexpressed blsecA, csaA, dnak, ffh, ftsY, groESL operon, prsA, qssecA, scr, SRP, and yrdF, respectively. The molecular weight of mature rhFGF21 is about 27 kDa. Column M: molecular weight marker. The strains 1A751C and CIS5 served as negative and positive controls