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. 2019 Jan 28;18:17. doi: 10.1186/s12934-019-1066-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — SDS-PAGE analysis of expression of six extracellular proteases deficient strain Kno6cf in culture medium following 24 h incubation. The molecular weight of mature rhFGF21 is about 25 kDa. Column M: molecular weight marker. The strain 1A751C served as negative control