Table 2.
Applied FISH probe panels for blood culture diagnostics
| Target organism | Probe sequence | Reference |
|---|---|---|
| Pan-eubacterial probe combined with all panels | ||
| All bacteria | 5'-GCT-GCC-TCC-CGT-TAG-GAG-T-3' | [27] |
| Panel for Gram-positive cocci in grape-like clusters | ||
| Staphylococcus aureus | 5'-GAA-GCA-AGC-TTC-TCG-TCC-G-3' | [3] |
| Staphylococcus spp. | 5'-TCC-TCC-ATA-TCT-CTG-CGC-3' | [3] |
| Micrococcus spp. | 5'-GTA-TCT-CTA-CGG-CGA-TCG-3' | This study |
| Panel for Gram-positive cocci in doubles or chains | ||
| Enterococcus faecalis | 5'-GAA-AGC-GCC-TTT-CAC-TCT-TAT-GC-3' | [4] |
| Enterococcus faecium | 5'-TTC-ACA-CAA-TCG-TAA-CAT-CCT-A-3' | [4] |
| Enterococcus gallinarum | 5'-ATT-CAC-AAC-TGT-GTA-ACA-TCC-TAT-3' | [4] |
| Enterococcus spp. | 5'-CAC-CGC-GGG-TCC-ATC-CAT-CA-3' and 5 '-CAG-TTC-TCT-GCG-TCT-ACC-TC-3' | [4] |
| Streptococcus agalactiae | 5'-GTA-AAC-ACC-AAA-CMT-CAG-CG-3' | [28] |
| Streptococcus pneumoniae | 5'-GTG-ATG-CAA-GTG-CAC-CTT-3' in combination with the competitor probe 5'-GTG-ATG-CAA-TTG-CAC-CTT-3' | [3] |
| Streptococcus pyogenes | 5'-CTA-ACA-TGC-GTT-AGT-CTC-TC-3' | This study |
| Streptococcus spp. | 5'-GTT-AGC-CGT-CCC-TTT-CTG-G-3' | [29] |
| Panel for Gram-negative rod-shaped bacteria | ||
| Acinetobacter spp. | 5'-TTA-GGC-CAG-ATG-GCT-GCC-3' | [6] |
| Bacteroides spp. | 5'-CAT-CCT-TCA-CGC-TAC-TTG-GCT-GG-3' in combination with the two competitor probes 5'-TCC-TTC-ACG-CGA-CTT-GGC-TGG-TT-3' and 5'-TCC-TGC-ACG-CTA-CTT-GGC-TGG-T-3' | This study |
| Bacteroides spp. / Prevotella spp. | 5'-CAT-CCT-TCA-CGC-TAC-TTG-GCT-GG-3' in combination with the competitor probe 5'-TCC-TTC-ACG-CGA-CTT-GGC-TGG-TT-3' | This study |
| Enterobacteriaceae | 5'-CCC-CCW-CTT-TGG-TCT-TGC-3' | [3] |
| Escherichia coli | 5'-GCG-GGT-AAC-GTC-AAT-GAG-CAA-AGG-3' | This study |
| Klebsiella pneumoniae | 5'-CCT-ACA-CAC-CAG-CGT-GCC-3' | [3] |
| Pseudomonas aeruginosa | 5'-CCA-CTT-TCT-CCC-TCA-GGA-CG-3' | This study |
| Salmonella spp. | 5'-TGC-GCT-TTT-GTG-TAC-GGG-GCT-3' in combination with the competitor probe 5'-GTG-CAT-TTT-TGT-GTA-CGG-GGC-3' and 5'-CTT-CAC-CTA-CGT-GTC-AGC-G-3' in combination with the competitor probe 5'-TCA-CCT-ACA-TAT-CAG-CGT-GC-3' | [7] |
| Stenotrophomonas maltophilia | 5'-GTC-GTC-CAG-TAT-CCA-CTG-C-3' | [26] |