Figure 1.
Tregs are efficiently targeted by Yptb for translocation of effector proteins. CD4+ T cells isolated from Foxp3hCD2 reporter mice were co-cultured with Yptb-WT-Bla at 37 °C for 1 h at MOI 35, labelled with CCF4-AM and subsequently analyzed by flow cytometry. Alive unmodulated cells are labelled in “Green”, and cells that were modulated by Yptb show “Blue” fluorescence. (a) Representative dot plots show frequency of translocation in the indicated T-cell subsets. Numbers indicate the frequency of modulated “Blue” cells in the corresponding gates. (b) Scatter-plot summarizes the frequency of modulated “Blue” cells among the indicated T-cell subsets. Data were pooled from 4 independent experiments (**p < 0.01; Bla, β-lactamase; MOI, multiplicity of infection)