Figure 4.

Attachment of Yptb to Tregs essentially requires invasins. Foxp3⁺ Tregs isolated from Foxp3^hCD2 reporter mice were co-cultured with Yptb-WT or Yptb-Δinv at 37 °C for 1 h at MOI 50. Yptb-WT without Tregs served as control. After repeated washing and centrifugation to remove unbound bacteria, the remaining cell pellet was plated in serial dilutions, and CFU per Treg were determined. “+” indicates the presence of Tregs in the co-cultures. Each dot represents a biological unicate. Data were pooled from 6 independent experiments (*p < 0.05; **p < 0.01; inv, invasin; MOI, multiplicity of infection; CFU, colony forming unit)