Table 1.

Newly designed LAMP primers for this study

Species	P. falciparum	P. knowlesi	P. vivax
Target gene	sal-1	sal-1	DHFR
Primer F3	5‘-AAG-CAA-CGG-ATT-GTC-CTG-3‘	5‘-GCA-TGG-TGT-AAT-CCT-CCA-G-3‘	5‘-AGA-ACT-GCA-ATG-CGT-TGA-AC-3‘
Primer B3	5‘-GCA-TCA-TCT-GTC-ATA-CCA-TCA-3‘	5‘-GAA-GCC-ACA-GAG-AAT-CCG-3‘	5‘-CCA-CAC-CTG-TTC-TGT-CTC-C-3‘
Primer forward inner primer (FIP) (F1c + F2)	5‘-GCA-ATC-CTC-CTG-ACC-AAT-TCA-CGC-TGG-AGC-TTC-GAT-AGA-TG-3‘	5‘-AGC-AGT-CGT-GTG-CAG-GTG-TTA-CAC-AGA-TAT-CAG-CAC-AGT-G-3‘	5‘-AAG-CTG-AAG-TAC-ACG-AGG-TCG-CGT-GGC-AGA-AGA-ACA-ACG-3‘
Primer backward inner primer (BIP) (B1c + B2)	5‘-ATG-GAG-ATG-GTG-TTG-AGG-AAG-CAC-ACC-AAC-ATC-CGT-AAT-ATC-TC-3‘	5‘-GAG-TCA-ACT-CGT-TCA-CAT-CAC-TCT-TCG-CTA-ATC-GTG-TCG-TAC-AA-3‘	5‘-CCG-GAG-CAC-CTG-CAA-GAT-TTG-ATG-ATG-TCG-TAT-ATG-ATG-CCT-AG-3‘
Primer LoopF	5‘-CAT-ATG-TCA-GCA-CAA-TGG-TGA-T-3‘	5‘-TAG-ATG-GAG-CTT-CTA-AGC-TGA-AC-3‘	5‘-TCT-TCC-TCT-GCC-TCG-G-3‘
Primer LoopB	5‘-ACA-CAT-CGT-GTT-ATG-ACA-GT-3‘	5‘-GTG-GTC-TGT-ACA-CCT-CCA-TG-3‘	5‘-CAA-CAG-CCT-GAA-GAT-TAA-GCA-G-3‘