Table 2.

LAMP reaction conditions on the Genie II Mk2 system (Amplex Diagnostics GmbH)

Software on the LAMP devices	eazyReport™-IVD-software (Amplex Diagnostics GmbH)
Master mix	Isothermal master mix ISO-001 (OptiGene Ltd., Horsham, UK)
Dilution of individual LAMP primers for the mixing of primer mix	100 pmol/μL
Proportions of primer dilutions of the primers	5 pmol + 5 pmol + 20 pmol + 20 pmol + 10 pmol + 10 pmol
F3 + B3 + BIP + FIP + LF + LB in the primer mix
Composition of LAMP reaction mix	25 μL containing 2 μL primer mix, 8 μL sample, and 15 μL isothermal master mix
Reaction time	40 min
Normalization threshold value	70,000 arbitrary units
Amplification threshold value	10,000 arbitrary units
Minimum ratio	0.025
Time of first measurement	3 min after initiation of the reaction