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. 2019 Jan 21;9:1. doi: 10.3389/fcimb.2019.00001

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Copyright © 2019 Li, Wang, Zhang, Li, Yan, Li, Jia and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of the amplification temperatures and amplification times on the LF-RPA assay. (A) The LF-RPA worked well in a wide range of amplification temperature from 25 to 45°C. (B) After 10min of isothermal amplification reaction, the positive reaction was visible on the test strip.