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. 2019 Jan;60(1):58–67. doi: 10.1165/rcmb.2018-0058OC

Figure 5.

Figure 5.

Inhibition of RV-A16 replication in BEAS-2B cells induced by C3m is independent of nitric oxide synthase (NOS) and GSNOR. (A) Neither l-nitroarginine methyl ester (l-NAME) (1 mM), a nonspecific NOS inhibitor, nor 1400W (100 μM), a specific NOS2 inhibitor, has a significant effect on RV-A16 RNA expression. n = 8/group; n = 3 replicates. **P < 0.001 by one-way ANOVA. (B) GSNOR protein expression (Western blot, upper panel) and activity (table, lower panel) in two different BEAS-2B clones (22 and 76) after GSNOR knockdown (GSNOR KD) or lentiviral transduction with scrambled RNA control (Scr). RT-qPCR quantification of (C) GSNOR and (D) RV-A16 RNA in BEAS-2B cells transduced with scrambled (Scr) or GSNOR-specific shRNA (KD) normalized to DMSO control (D) and scrambled (Scr) DMSO control (E). n = 6/group; n = 2 replicates. *P < 0.0001 by paired Student’s t test.