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. 2019 Jan;60(1):117–127. doi: 10.1165/rcmb.2018-0105OC

Figure 1.

Figure 1.

Yes-associated protein (YAP) 1 controls Tie2 expression in human lung microvascular endothelial (L-HMVE) cells. (A) Graph showing YAP1 and Tie2 mRNA levels in L-HMVE cells treated with YAP1 siRNA #1 or control siRNA with irrelevant sequences (n = 3, *P < 0.05). (B) Immunoblots showing YAP1, Tie2, and β-actin protein levels in L-HMVE cells treated with YAP1 siRNA #1 or control siRNA with irrelevant sequences (left). Graph showing YAP1 and Tie2 protein levels in L-HMVE cells treated with YAP1 siRNA #1 or control siRNA with irrelevant sequences (right, n = 3, *P < 0.05). (C) Graph showing YAP1 and Tie2 mRNA levels in L-HMVE cells treated with lentivirus encoding full-length YAP1 or YAP1S127A mutant construct (n = 3, *P < 0.05). (D) Immunoblots showing YAP1S127 phosphorylation and Tie2 and β-actin protein levels in L-HMVE cells treated with lentivirus encoding full-length YAP1 or YAP1S127A mutant construct (left). Graph showing Tie2 protein levels in L-HMVE cells treated with lentivirus encoding full-length YAP1 or YAP1S127A mutant construct (right, n = 3, *P < 0.05). (E) Graph showing TEA domain transcription factor (TEAD) 1 and Tie2 mRNA levels in L-HMVE cells treated with TEAD1 siRNA #1 or control siRNA with irrelevant sequences (n = 3, *P < 0.05). (F) Immunoblots showing TEAD1, Tie2, and β-actin protein levels in L-HMVE cells treated with TEAD1 siRNA #1 or control siRNA with irrelevant sequences (left). Graph showing the quantification of immunoblots (right, n = 3, *P < 0.05). Error bars represent SEM.