Skip to main content
. 2019 Jan;60(1):117–127. doi: 10.1165/rcmb.2018-0105OC

Figure 6.

Figure 6.

Endothelial YAP1 is required for post-PNX compensatory lung growth and angiogenesis. (A) Graph showing the ratio of the weight of right lung cardiac lobe to mouse BW 7 days after left PNX in Yap1fl/fl-Cdh5(PAC)-CreERT2 or control Yap1fl/fl mice (n = 7, mean ± SEM, *P < 0.05). (B) Micrographs showing blood vessel structures in the mouse right lung lobe 7 days after left PNX analyzed using the Microfil casting system (top row; scale bar: 1 mm). H&E–stained cardiac lobe of Yap1fl/fl-Cdh5(PAC)-CreERT2 or Yap1fl/fl mice 7 days after PNX (second row; scale bar: 25 μm). Immunofluorescence micrographs showing TEAD1 (third row) and Tie2 expression (bottom row) in the Yap1fl/fl-Cdh5(PAC)-CreERT2 or control Yap1fl/fl mouse lungs 7 days after PNX (scale bar: 25 μm). Graphs showing the quantification of blood vessel density (right first) and the number of branching points (right second) in the microfil-casted lungs, and alveolar number (right third) and alveolar size (MLI, right fourth) in the H&E-stained lungs (n = 7, mean ± SEM, *P < 0.05). (C) IB showing YAP1, Tie2, TEAD1, and β-actin protein levels in ECs isolated from Yap1fl/fl-Cdh5(PAC)-CreERT2 or control Yap1fl/fl mouse lungs 7 days after PNX.