Fig. 1.

Effects of S42 or DHT on Ar expression on C2C12 myotubes. C2C12 myotubes were incubated with 1–10 µM of S42 or 100 nM of DHT or appropriate vehicle (DMSO) for 24 h. (A) Comparison of mRNA expression levels of Ar relative to those of Gapdh by qPCR. Data are expressed as mean ± SE of triplicate samples. (B)Western blot analysis showing Ar and Gapdh. (C) Statistical comparison of the expression levels of Ar relative to Gapdh by Western blot analysis. Data are expressed as mean ± SE of triplicate samples. In statistical comparisons in (A) and (C), the data of treated groups with DHT or S42 were compared with that of untreated group. **P < 0.01 vs DMSO by one-way ANOVA.