Fig. 3.

S42 increases phosphorylation of p70S6K (Thr389) in C2C12 cells. Effects of S42 or insulin on phosphorylation of p70S6K (p-p70S6K) on C2C12 myotubes by Western blot analysis (A) and their statistical evaluations (B). C2C12 myotubes were incubated with 1–10 μM of S42 or 2 μM of insulin or appropriate vehicle for 24 h. Effect of S42 or insulin on phosphorylation of p70S6K (p-p70S6K) on C2C12 myotubes in the presence or absence of rapamycin by Western blotting (C) and their statistical evaluations (D). C2C12 myotubes were incubated with 1–10 μM of S42 or 2 μM of insulin or appropriate vehicle in the presence or absence of 1 nM of rapamycin for 24 h. In statistical comparisons, expressions of p-p70S6K protein relative to p70S6K protein were determined and the data of treated groups with S42 or insulin were compared with that of untreated group. Data are expressed as mean ± SE of triplicate samples. ^*P < 0.05, ^**P < 0.01 vs DMSO, ^# P < 0.05, ^##P < 0.01 in indicated comparisons by one-way ANOVA. ns, not significant.