Fig. 4.

Effects of S42 on phosphorylation of Akt (Ser473) or Erk on C2C12 myotubes by Western blot analysis (A, B) and their statistical evaluations (C, D). C2C12 myotubes were incubated with 1–10 μM of S42 or 2 μM of insulin or appropriate vehicle for 24 h. In statistical comparisons, expressions of p-Akt relative to Akt (B) or p-Erk relative to Erk (D) were determined. Data of treated groups with S42 or insulin were compared with those of untreated group. Data are expressed as mean ± SE of triplicate samples. ^**P < 0.01 vs DMSO by one-way ANOVA.