Figure 2.

WFDC2 Is Under Hypoxia Regulation Directly through the HRE in Its Promoter

(A) Consensus sequence for the hypoxia response element (HRE) was identified in the promoter region of WFDC2. Wild-type (HREwt-Luc) and mutated (HREmut-Luc) sequences from the WFDC2 promoter were respectively cloned at the 5′ of luciferase reporter open reading frame (Luc). (B) Binding of HIF1α to the HRE in the WFDC2 promoter was analyzed by ChIP assay, using control antibody (Ab) and HIF1α Ab, respectively. (C) Luciferase activities of HREwt-Luc and HREmut-Luc constructs were measured in GC cells incubated in media containing 0 μM (normoxia) or 100 μM (hypoxia) CoCl₂, respectively. Data are presented as mean ± SD. *p < 0.05, control versus HIF1α Ab; **p < 0.01; ns, not significant, normoxia versus hypoxia.