Effect of HIF1α Overexpression on HE4 Level in GC Cells
(A) Expressions of WFDC2 mRNA were assessed in GC cell lines SGC-7901 and MKN-49P stably expressing control or WFDC2 shRNA, bearing either empty vector or HIF1α overexpression (OE), respectively. (B) Expressions of HE4 and HIF1α proteins were assessed in GC cell lines SGC-7901 and MKN-49P stably expressing control or WFDC2 shRNA, bearing either empty vector or HIF1α OE, respectively. Data are presented as mean ± SD. *p < 0.05, and **p < 0.01, HIF1α OE + control versus empty vector + control and HIF1α OE + WFDC2 shRNA.